POTENTIAL of Caulerpa lentillifera EXTRACT IN ENHANCING HEMOCYTE AND GROWTH BOOSTER IN SPINY LOBSTER (PANULIRUS HOMARUS)
Abstract
Challenges faced by lobster farmers in Indonesia include the high mortality rate of lobster larvae and the lengthy cultivation period. In previous studies, we successfully enhanced the immune system and growth of fish and shrimp using Caulerpa lentillifera extract. This study aims to analyze the effects of Caulerpa lentillifera extract on survival rate, growth, and immune system in Panulirus homarus larvae. The research method was experimental, using a Randomized Block Design (RBD) with treatments: control, Treatment 1 (10 gr/kg), and Treatment 2 (20 gr/kg), each with three replications over one month. The parameters observed were survival rate, growth rate, specific growth rate, total hemocyte count, and differential hemocyte count. The results indicated that Caulerpa lentillifera extract successfully improved survival rate and growth, as evidenced by increased survival rate, growth rate, specific growth rate, and an interesting food conversion ratio of around 1, with the best results in Treatment 2. Furthermore, Caulerpa lentillifera extract enhanced the immune system by increasing total hemocyte count and differential leukocytes (granulocytes, semi-granulocytes, and hyaline cells), with the highest values observed in Treatment 2.
References
Anand P. S. S., Balasubramanian C. P., Christina L., Kumar S., Biswas G., De D., Ghoshal T. K., Vijayan K. K. (2019). Substrate based black tiger shrimp, Penaeus monodon culture: Stocking density, aeration and their effect on growth performance, water quality and periphyton development. Aquaculture 507:411-418.
Arzad, M., Ratna, R., & Fahrizal, A. (2019). Pengaruh padat tebar terhadap pertumbuhan ikan nila (oreochromis niloticus) dalam sistem akuaponik. Median Jurnal Ilmu Ilmu Eksakta, 11(2), 39-47.https://doi.org/10.33506/md.v11i2.503
Brandt, A., Grikscheit, K., Siede, R., Grosse, R., Meixner, M., & Büchler, R. (2017). Immunosuppression in honeybee queens by the neonicotinoids thiacloprid and clothianidin. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-04734-1
Cui, C., Tang, X., Xing, J., Sheng, X., Chi, H., & Zhan, W. (2022). Single-cell rna-seq uncovered hemocyte functional subtypes and their differentiational characteristics and connectivity with morphological subpopulations in litopenaeus vannamei. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.980021
Destoumieux, D., Saulnier, D., Garnier, J., Jouffrey, C., Bulet, P., Bachere, E. (2001). Antifungal peptides are generated from the C terminus of shrimp hemocyanin in response to microbial challenge. J. Biol. Chem. 276, 47070–47077
Desy Windia Yuniarti, Maftuch and Muhammad Fadjar. 2015. Application of Immunostimulants from Caulerpa racemosa Extract to Improve Immune Response of Giant Gourami Fish (Osphronemous Gouramy) to Aeromonas hydrophila Infection. J. Life Sci. Biomed. 5(3): 60-64, May 30, 2015
Er, A., Taşkıran, D., & Sak, O. (2017). Azadirachtin-induced effects on various life history traits and cellular immune reactions of galleria mellonella (lepidoptera: pyralidae). Archives of Biological Sciences, 69(2), 335-344. https://doi.org/10.2298/abs160421108e
Fan, X. and Zhang, X. (2023). Hallmarks of crustacean immune hemocytes at single-cell resolution. Frontiers in Immunology, 14. https://doi.org/10.3389/fimmu.2023.1121528
Hertika Et Al, A. M. S. (2023). Effect of Ethanol and Methanol Extract of Caulerpa lentilifera on Hematological Parameters and Phagocytosis of Cyprinus carpio. Egyptian Journal of Aquatic Biology and Fisheries, 27(1), 385-395.
Hertika Et Al, A. M. S. (2024). The Growth and Hemocyte Analysis of the Shrimp (Litopenaeus vannamei) Induced by Different Concentration of Artificial Seawater and Water Extract Of Sea Grapes (Caulerpa lentillifera). Egyptian Journal of Aquatic Biology and Fisheries, 28(2), 859-869.
Hertika, A. M. S., Supriatin, F. E., & Putra, R. B. D. S. (2024). The Potential Antibacteria Activity of Variety Extract of Caulerpa lentillifera (ethanol, methanol, water extract) Againts Vibrio sp. (Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus). JFMR (Journal of Fisheries and Marine Research), 8(1), 29-38.
Hong, M., Hwang, D., & Cho, S. (2018). Hemocyte morphology and cellular immune response in termite (reticulitermes speratus). Journal of Insect Science, 18(2). https://doi.org/10.1093/jisesa/iey039
Hong, X., Wang, Y., Wang, K., Wei, C., Li, W., Yu, L., … & Liu, X. (2023). Single-cell atlas reveals the hemocyte subpopulations and stress responses in asian giant softshell turtle during hibernation. Biology, 12(7), 994. https://doi.org/10.3390/biology12070994
Kumar, M., Gupta, V., Kumari, P., Reddy, C. R. K., & Jha, B. (2011). Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. Journal of Food Composition and Analysis, 24(2), 270–278. https://doi.org/10.1016/j.jfca.2010.07.007
Lin, Y., Maaroufi, H., Ibrahim, E., Kučerová, L., & Z̆urovec, M. (2019). Expression of human mutant huntingtin protein in drosophila hemocytes impairs immune responses. Frontiers in Immunology, 10. https://doi.org/10.3389/fimmu.2019.02405
Liu, C. H., Yeh, S. T., Cheng, S. Y., & Chen, J. C. (2004). The immune response of the white shrimp Litopenaeus vannamei and its susceptibility to Vibrio infection in relation with the moult cycle. Fish & shellfish immunology, 16(2), 151-161.
Madusari, B. D., Ariadi, H., & Mardhiyana, D. (2022). Effect of the feeding rate practice on the white shrimp (Litopenaeus vannamei) cultivation activities. Aquaculture, Aquarium, Conservation & Legislation-International Journal of the Bioflux Society, 15(1), 473-479.
Mao, F., Wong, N., Lin, Y., Zhang, X., Liu, K., Huang, M., … & Yu, Z. (2020). Transcriptomic evidence reveals the molecular basis for functional differentiation of hemocytes in a marine invertebrate, crassostrea gigas. Frontiers in Immunology, 11. https://doi.org/10.3389/fimmu.2020.00911
Martins, Á., Flores, J., Porto, C., Romano, L., Wasielesky, W., Caldas, S., … & Monserrat, J. (2018). Antioxidant effects of nanoencapsulated lipoic acid in tissues and on the immune condition in haemolymph of pacific white shrimplitopenaeus vannamei (boone, 1931). Aquaculture Nutrition, 24(4), 1255-1262. https://doi.org/10.1111/anu.12663
Muralisankar, T., Kalaivani, P., Thangal, S. H., & Santhanam, P. (2021). Growth, biochemical, antioxidants, metabolic enzymes and hemocytes population of the shrimp Litopenaeus vannamei exposed to acidified seawater. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 239, 108843.
Pondeville, E., Puchot, N., Parvy, J., Carissimo, G., Poidevin, M., Waterhouse, R., … & Bourgouin, C. (2019). Hemocyte-targeted gene expression in the female malaria mosquito using thehemolectinpromoter fromdrosophila. https://doi.org/10.1101/2019.12.13.875518
Powell, C. D., Tansil, F., France, J., & Bureau, D. P. (2020). Growth trajectory analysis of Pacific whiteleg shrimp (Litopenaeus vannamei): Comparison of the specific growth rate, the thermal‐unit growth coefficient and its adaptations. Aquaculture Research, 51(2), 480-489.
Prates, E., Holanda, M., Pedrosa, V. F., Monserrat, J. M., & Wasielesky, W. (2023). Compensatory growth and energy reserves changes in the Pacific white shrimp (Litopenaeus vannamei) reared in different temperatures and under feed restriction in biofloc technology system (BFT). Aquaculture, 562, 738821.
Putra, D. F., Abbas, M. A., Solin, N., & Othman, N. 2022. Effects of Dietary Caulerpa lentillifera Supplementation On Growth Performance and Survival Rate of Milk Fish, Chanos chanos (Forsskål, 1775). Elkawnie: Journal of Islamic Science and Technology, 7(2), 395-407.
Putra, D. F., Rahmawati, M., Abidin, M. Z., & Ramlan, R. 2019. Dietary administration of sea grape powder (Caulerpa lentillifera) effects on growth and survival rate of black tiger shrimp (Penaeus monodon). In IOP Conference Series: Earth and Environmental Science (Vol. 348, No. 1, p. 012100). IOP Publishing
Ridhowati, S., & Asnani. (2016). Potensi Anggur Laut Kelompok Caulerpa racemosa sebagai Kandidat Sumber Pangan Fungsional Indonesia. Oseana, 41(4), 50–62.
Santos, A., Botelho, M., Joviano, W., Gomes, V., Silva, J., & Umbuzeiro, G. (2023). Characterization of hemocytes from the marine amphipod parhyale hawaiensis (dana 1853): setting the basis for immunotoxicological studies. Invertebrate Biology, 142(1). https://doi.org/10.1111/ivb.12394
Saputri, A. U., Purnamayati, L., & Anggo, A. D. (2019). Aktivitas Antibakteri Anggur Laut (Caulerpa lentillifera) Terhadap Staphylococcus aureusdan Escherichia coli. Jurnal Ilmu Dan Teknologi Perikanan, 1(1), 15–20.
Sritunyalucksana, K., W. Gangnonngiw, S. Archakunakorn, D. Fegan, T.W. Flegel. (2005). Bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimp. Dis. Aquat. Org. 63, 89–94.
Triastini, N., Muskita, W., & Susilowati, P. (2017). Substitusi tepung kedelai (glycine max) dengan tepung bungkil biji kapuk (ceiba petandra) hasil fermentasi dalam pakan buatan terhadap pertumbuhan dan kelangsungan hidup juvenil udang vaname (litopenaeus vannamei). Jsipi (Jurnal Sains dan Inovasi Perikanan) (Journal of Fishery Science and Innovation), 1(2). https://doi.org/10.33772/jsipi.v1i2.6625
Vanha-aho, L., Ines, A., Vesala, L., Hultmark, D., Valanne, S., & Rämet, M. (2015). Edin expression in the fat body is required in the defense against parasitic wasps in drosophila melanogaster. Plos Pathogens, 11(5), e1004895. https://doi.org/10.1371/journal.ppat.1004895
Wang, C., Wang, Z., Kariuki, M., Ling, Q., Kiguchi, K., & Ling, E. (2010). Physiological functions of hemocytes newly emerged from the cultured hematopoietic organs in the silkworm, bombyx mori. Insect Science, 17(1), 7-20. https://doi.org/10.1111/j.1744-7917.2009.01288.x
Yan, Y., Sigle, L., Rinker, D., Estévez-Lao, T., Capra, J., & Hillyer, J. (2022). The imd and jnk pathways drive the functional integration of the immune and circulatory systems of mosquitoes.. https://doi.org/10.1101/2022.01.26.477938
Zhang, Q., Huang, J., Zhu, J., & Yè, G. (2011). Parasitism of pieris rapae (lepidoptera: pieridae) by the endoparasitic wasp pteromalus puparum (hymenoptera: pteromalidae): effects of parasitism on differential hemocyte counts, micro‐ and ultra‐structures of host hemocytes. Insect Science, 19(4), 485-497. https://doi.org/10.1111/j.1744-7917.2011.01454.x
Zhang, W., Chen, J., Keyhani, N., Zhang, Z., Li, S., & Xia, Y. (2015). Comparative transcriptomic analysis of immune responses of the migratory locust, locusta migratoria, to challenge by the fungal insect pathogen, metarhizium acridum. BMC Genomics, 16(1). https://doi.org/10.1186/s12864-015-2089-9


















